Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Accutase solution, MFCD01632905, 100mL
Accutase solution (in dulbecco′s phosphate-buffered saline (DPBS) without Ca++ or Mg++) contains proteolytic and collagenolytic enzymes for cell detachment. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
Zymo Research Corporation RNASE A, 30 mg
RNase A specifically cleaves at the 3’-side of pyrimidine (uracil or cytosine) phosphate bonds. The enzyme does not hydrolyze DNA, because DNA lacks 2’-OH groups essential for the formation of cyclic intermediates. The enzyme can also be used to hydrolyze RNA from protein samples. It is compatible for use in RNase protection assays, to remove unspecifically bound RNA,in the analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and in the purification of DNA.
Takara Bio CRYONASE COLD-ACTIVE NUCLEASE
Enzyme; Cryonase Cold-active Nuclease; Takara; 10,000U (20 U/uLx500 uL); Source: Purified from E. coli; Purity: Protease activity is less than detection limit; Storage: -20 deg.C
Takara Bio TAQ HOT START PREMIX
PCR Reagents; Takara; Hot-Start Taq DNA Polymerase; 100rxns; reduces background, increased specificity, permits room-temperature reaction assembly
New England Biolabs, Inc. Vaccinia Capping System – 400 units
Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, and translation of mRNAs. Enzymatic production of capped RNA is an easy way to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection. Alternatively, use of labeled GTP in a reaction provides a convenient way to label any RNA containing a 5' terminal triphosphate. Vaccinia capping enzyme is composed of two subunits (D1 and D12). The D1 subunit carries three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase); all necessary for addition of a complete Cap 0 structure, m7Gppp5'N to 5' triphosphate RNA. In vitro transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and the methyl donor, SAM.
New England Biolabs, Inc. Cre Recombinase – 250 units
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products depend on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused. DNA between directly repeated loxP sites will be excised in circular form while DNA between opposing loxP sites will be inverted with respect to external sequences.
Sigma Aldrich Fine Chemicals Biosciences Liberase(TM) TM Research Grade medium Thermolysin concentration |
Liberase(TM) TM Research Grade medium Thermolysin concentration |
United States Biological Corporation ZYMOLYASE 20T
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg
Enzo Life Sciences Matrix metalloproteinase (MMP) multipack-2 (1 Kit)
The MMP multipack-2 contains 10µg each of five highly active recombinant MMP catalytic domains: MMP-3, MMP-7, MMP-10, MMP-11, and MMP-12. Contents: Contains 10µg of each enzyme, provided in a screw-cap microfuge tube. MMP-3 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE109), MMP-7 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE181), MMP-10 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE392), MMP-11 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE282), MMP-12 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE138). Application: FUNC, HTS. Long Term Storage: -80°C
Sigma Aldrich Fine Chemicals Biosciences Pepsin, 1g
Pepsin is an aspartic endoproteinase isolated from pig gastric mucosa. It is mainly used for the unspecific hydrolysis of proteins and peptides in acidic media. It also provides a limited hydrolysis of native immunoglobulins to yield biologically active fragments.
New England Biolabs, Inc. Afu Uracil-DNA Glycosylase (UDG) – 200 units
A thermostable homolog of the E. coli Uracil-DNA Glycosylase (UDG) from Archaeoglobus fulgidus. Afu UDG catalyzes the release of free uracil from uracil-containing DNA. Afu UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA.
New England Biolabs, Inc. Endoproteinase AspN – 50 µg
Endoproteinase AspN (flavastacin) is a zinc metalloendopeptidase which selectively cleaves protein and peptide bonds N-terminal to aspartic acid residues.
New England Biolabs, Inc. T4 RNA Ligase 2 – 150 units
T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity). Unlike T4 RNA Ligase 1 , T4 RNA Ligase 2 is much more active joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5 phosphate and 3' OH for ligation. The enzyme can also ligate the 3' OH of RNA to the 5 phosphate of DNA in a double stranded structure.
New England Biolabs, Inc. ZraI – 1000 units
A restriction endonuclease that recognizes the sequence GAC_^GTC.
New England Biolabs, Inc. TelN Protelomerase – 250 units
TelN Protelomerase, from phage N15, cuts dsDNA at a TelN recognition sequence (56 bp) and leaves covalently closed ends at the site of cleavage.